Objective: Lindane is an organochlorine pesticide used for pharmaceutical and agricultural purposes. The aim of the present study was to investigate the biosorption of lindane from aqueous solutions using pretreated dried biomass of anamorphous yeast, Cintractia sorghi VITJzN02 isolated from contaminated soil. Methods: The characteristics of surface modified yeast biomass during lindane biosorption were investigated by batch equilibrium studies, FTIR and SEM analysis. Results: Among the various pretreated chemicals tested for surface modifications, citric acid showed the best result for enhanced uptake of lindane. Lindane biosorption was most favoured at acidic pH and sorption equilibrium was attained in 330 min with an initial lindane concentration 500 mg/l. Modified biomass were found to have 3-4 folds higher biosorption yield compared to dried natural biomass. Adsorption isotherms were best fitted to the Langmuir model showing monolayer sorption capacity 100 mg/g. Four kinetic models viz. pseudo-first-order, pseudo-second-order, intra-particle diffusion and Elovich were employed to explain the sorption mechanism. The kinetic data were found to follow the pseudo first order kinetic model suggesting that adsorption process was controlled by physisorption. The intra-particle diffusion plots being non linear over whole time range indicated that intra particle diffusion was not the only rate limiting step for biosorption of lindane. Conclusions: Biosorption of lindane by pretreated biomass of yeast Cintractia sorghi VITJzN02 is governed by physisorption and monolayer adsorption pattern. Based on the results, it may be concluded that biosorbent prepared from yeast Cintractia sorghi VITJzN02 can serve as potential adsorbent for the removal of lindane from aqueous solutions.