The aim of the present study was actinomycetes mediated biosynthesis of copper oxide nanoparticles (CuO NPs) and evaluation of its antibacterial activity against selected human and fish pathogens. The biosynthesized CuO NPs were characterized by UV–Visible (UV-Vis) spectroscopy. The presence of capping agents over the metal nanoparticles was confirmed by Fourier-Transform Infrared Spectroscopy (FT-IR). The crystalline nature of the CuO NPs was illustrated by X- Ray diffractometer (XRD). The average size of the biosynthesized CuO NPs from XRD and Transmission Electron Microscopy (TEM) was 61.7 nm. The XRD and Energy Dispersive X-Ray spectroscopy (EDX) suggests the purity of the biosynthesized CuO NPs. The morphology and size was viewed under Scanning Electron Microscopy (SEM). The Dynamic Light Scattering (DLS) results provided the zeta potential of − 31.1 mV which further confirmed the stability of the CuO NPs. The biosynthesized CuO NPs showed higher antibacterial activity (zone of inhibition) against various human and fish bacterial pathogens (Staphylococcus aureus, Bacillus cereus, Proteus mirabilis, Edwardsiella tarda, Aeromonas caviae, Aeromonas hydrophila and Vibrio anguillarum). The antibacterial activity of CuO NPs was significantly higher than the activity exhibited by cell free supernatant of actinomycetes. Among the pathogens tested B. cereus was more susceptible (25.3 mm) to biosynthesized CuO NPs. The antibacterial activity exhibited by the actinomycetes mediated biosynthesized CuO NPs suggests that it can combat both human as well as fish bacterial pathogens. To the best of our knowledge, this is the first report on actinomycetes mediated biosynthesis of CuO NPs. © 2018 Elsevier Ltd

Kannabiran K

Department of Bio-Medical Sciences

School of Bio Sciences and Technology

Vellore Campus

Nabila M.I

Vellore Institute of Technology (VIT) is a private university located in&nbsp;Tamil Nadu, India. Founded in 1984, as Vellore Engineering College, the institution offers 20 undergraduate, 34 postgraduate, four integrated and four research programs. It has campuses in Vellore, Amravati, Bhopal and Chennai.

VIT is one of the top ranked private universities in India according to NIRF, THE and QS Rankings.&nbsp;Govt. of India has recognized&nbsp;VIT, Vellore as an&nbsp;Institution of Eminence. This has allowed VIT to take independent quality initiatives and move up in world ranking.

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VIT University

Biocatalysis and Agricultural Biotechnology

Environment friendly methods for the synthesis of copper nanoparticles have become a valuable trend in the current scenario. The utilization of phytochemicals from plant extracts has become a unique technology for the synthesis of nanoparticles, as they possess dual nature of reducing and capping agents to the nanoparticles. In the present investigation we have synthesized copper nanoparticles (CuNPs) using a rare medicinal plant Cissus arnotiana and evaluated their antibacterial activity against gram negative and gram positive bacteria. The morphology and characterization of the synthesized CuNPs were studied and done using UV-Visible spectroscopy at a wavelength range of 350–380 nm. XRD studies were performed for analyzing the crystalline nature; SEM and TEM for evaluating the spherical shape within the size range of 60–90 nm and AFM was performed to check the surface roughness. The biosynthesized CuNPs showed better antibacterial activity against the gram-negative bacteria, E. coli with an inhibition zone of 22.20 ± 0.16 mm at 75 μg/ml. The antioxidant property observed was comparatively equal with the standard antioxidant agent ascorbic acid at a maximum concentration of 40 μg/ ml. This is the first study reported on C. arnotiana mediated biosynthesis of copper nanoparticles, where we believe that the findings can pave way for a new direction in the field of nanotechnology and nanomedicine where there is a significant potential for antibacterial and antioxidant activities. We predict that, these could lead to an exponential increase in the field of biomedical applications, with the utilization of green synthesized CuNPs, due to its remarkable properties. The highest antibacterial property was observed with gram-negative strains mainly, E. coli, due to its thin peptidoglycan layer and electrostatic interactions between the bacterial cell wall and CuNPs surfaces. Hence, CuNPs can be potent therapeutic agents in several biomedical applications, which are yet to be explored in the near future. © 2019 Elsevier B.V.

Fulltext

Journal of Photochemistry and Photobiology B: Biology

Antibacterial and antioxidant potential of biosynthesized copper nanoparticles mediated through Cissus arnotiana plant extract

Recently, non-toxic source mediated synthesis of metal and a metal oxide nanoparticle attains more attention due to key applicational responsibilities. This present report stated that the eco-friendly synthesis of copper oxide nanoparticles (CuO NPs) using Cissus quadrangularis (C. quadrangularis) plant extract. Further the eco-friendly synthesized CuO NPs were characterized using a number of analytical techniques. The observed results stated that the synthesized CuO NPs were spherical in shape with 30±2nm. Then the eco-friendly synthesized CuO NPs were subjected for anti-fungal against two strains namely Aspergillus niger (A. niger) resulted in 83% at 500ppm, 86% of inhibition at 1000ppm and Aspergillus flavus (A. flavus) resulted in 81% at 500ppm, 85% of inhibition at 1000ppm respectively. Despite the fact that compared to standard Carbendazim, eco-friendly synthesized CuO NPs exhibits better results were discussed in this manuscript.

Materials Science and Engineering: C

Cissus quadrangularis mediated ecofriendly synthesis of copper oxide nanoparticles and its antifungal studies against Aspergillus niger, Aspergillus flavus

The eco-friendly synthesis of nanoparticles through green route from plant extracts have renowned a wide range of application in the field of modern science, due to increased drug efficacy and less toxicity in the nanosized mediated drug delivery model. In the present study, our research groups have biosynthesized the stable and cost effective copper oxide nanoparticles (CuO NPs) from the leaves of (Ormocarpum cochinchinense) O. cochinchinense. The synthesis of crystalline CuO NPs from the leaf extract of O. cochinchinense were confirmed by various analytical techniques like UV-Visible Spectroscopy (UV-Vis), Fourier-Transform Infrared Spectroscopy (FT-IR), X-Ray Diffractometer (XRD), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM) and Selected Area Electron Diffraction (SAED) pattern. Further the synthesized CuO NPs were screened for anticancer activity on human colon cancer cell lines (HCT-116) by MTT (3-(4,5-dimethyl-2-tiazolyl)-2,5-diphenyl-2-tetrazolium bromide) assay. The obtained result inferred that the synthesized CuO NPs demonstrated high anticancer cytotoxicity on human colon cancer cell lines (HCT-116) with IC50 value of 40μgmL-1 were discussed briefly in this manuscript.

Biosynthesis and characterization of copper oxide nanoparticles and its anticancer activity on human colon cancer cell lines (HCT-116)

Marine bacteria are considered as imperative source for novel antibacterial compounds. The marine Streptomyces species was isolated from the sediment sample collected at the coast of Andaman and Nicobar Islands, India and identified as Streptomyces sp. VITAK1. The petroleum ether (PE) extract prepared from the culture supernatant of the isolate was purified by silica gel column chromatography using a mixture of PE and ethyl acetate (1 : 1 vol/vol) as solvent system. The purified compound was identified by using 1H and 13C NMR spectral data and GC-MS based on the similarity index with the reference compounds available in the mass spectra library of National Institute for Standards and Technology as coumarin-6-ol, 3,4-dihydro-4,4,5,7-tetramethyl- (CDTM). The antibacterial activity of the purified compound was tested according to the National Committee for Clinical Laboratory Standards guidelines and it was revealed that the lead compound showed IC50 value ranging from 2.5–40 µg/mL against tested bacterial pathogens. Proteus vulgaris was the most susceptible (IC50: 2.5 µg/mL) to inhibition by CDTM. In addition, the lead compound showed concentration-dependent anticoagulant activity with human blood samples. The results of the study suggest that Streptomyces sp. VITAK1 is the potential source for coumarin type of antibacterial compound. © 2015, Pleiades Publishing, Inc.

Applied Biochemistry and Microbiology

Extraction and identification of antibacterial compound from marine Streptomyces sp. VITAK1 isolated from the coast of Andaman and Nicobar Islands, India

Journal	Data powered by TypesetBiocatalysis and Agricultural Biotechnology
Publisher	Data powered by TypesetElsevier BV
ISSN	1878-8181
Open Access	0