Pragasam V

Department of Bio-Sciences

School of Bio Sciences and Technology

Vellore Campus

Rimer J.D

Kolbach A.M

Ward M.D

Kleinman J.G

Wesson J.A.

Fulltext

Vellore Institute of Technology (VIT) is a private university located in&nbsp;Tamil Nadu, India. Founded in 1984, as Vellore Engineering College, the institution offers 20 undergraduate, 34 postgraduate, four integrated and four research programs. It has campuses in Vellore, Amravati, Bhopal and Chennai.

VIT is one of the top ranked private universities in India according to NIRF, THE and QS Rankings.&nbsp;Govt. of India has recognized&nbsp;VIT, Vellore as an&nbsp;Institution of Eminence. This has allowed VIT to take independent quality initiatives and move up in world ranking.

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VIT University

Urological Research

The study explores calcium oxalate crystal inhibiting characteristic of polyacrylic acid (pAA), an anionic polymer in in vitro and in vivo. Animals were divided into 5 groups where group 1 served as control, group 2 were made hyperoxaluric by supplementing with Ethylene glycol (EG) 0.75% (v/v) for 30 days. Group 3, 4 &amp; 5 were also given with EG and treated simultaneously with 2.5, 5 &amp; 10 mg of pAA/kg of body weight, respectively. Urine, serum and tissue analyses along with histological studies were performed at the end of the 30 days study. In vitro crystallization was significantly inhibited by pAA and further it was supported by particle size analyses, XRD and FT-IR studies. Toxicological analyses showed that pAA was safe to use in animals at concentrations below 100 mg/kg BW. In vivo anti-urolithic study showed significant improvement in urinary lithogenic factors (calcium, oxalate, phosphate, citrate &amp; magnesium) and renal function parameters (creatinine, urea and protein). Tissue analyses on anti-oxidant enzyme activity and lipid peroxides showed maintenance of tissue antioxidant status in the pAA supplemented rats and histological studies demonstrated the nephroprotection offered by pAA and were concurrent to the biochemical analyses. Supplementation of pAA not only reduces the crystal aggregation but also regulates the expression and localization of crystal inhibiting proteins and gene expression of inflammatory cytokines in experimental animals. In summary, pAA is a potent anti-urolithic agent in rats and we can propose that 10 mg/kg body weight is the effective dosage of pAA and this concentration can be used for further studies. © 2016 Elsevier Ireland Ltd.

Chemico-Biological Interactions

Polyacrylic acid attenuates ethylene glycol induced hyperoxaluric damage and prevents crystal aggregation in vitro and in vivo

Calcium oxalate monohydrate aggregation induced by aggregation of desialylated Tamm-Horsfall protein

American Journal of Physiology-Renal Physiology

Proteomic analysis of renal calculi indicates an important role for inflammatory processes in calcium stone formation

Journal of Endourology

Second Prize:Comprehensive Proteomic Analysis of Human Calcium Oxalate Monohydrate Kidney Stone Matrix

Nucleic Acids Research

The Universal Protein Resource (UniProt)

Renal calcinosis and stone formation in mice lacking osteopontin, Tamm-Horsfall protein, or both

Journal	Data powered by TypesetUrological Research
Publisher	Data powered by TypesetSpringer Science and Business Media LLC
ISSN	0300-5623
Open Access	Yes