Jayashree J

Department of Database Systems

School of Computer Science and Engineering

Vellore Campus

Bhaskara Rao K-V

Department of Bio-Medical Sciences

School of Bio Sciences and Technology

Shivani R

Anwesha S

I A.E.

Fulltext

Vellore Institute of Technology (VIT) is a private university located in&nbsp;Tamil Nadu, India. Founded in 1984, as Vellore Engineering College, the institution offers 20 undergraduate, 34 postgraduate, four integrated and four research programs. It has campuses in Vellore, Amravati, Bhopal and Chennai.

VIT is one of the top ranked private universities in India according to NIRF, THE and QS Rankings.&nbsp;Govt. of India has recognized&nbsp;VIT, Vellore as an&nbsp;Institution of Eminence. This has allowed VIT to take independent quality initiatives and move up in world ranking.

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VIT University

Journal of Infection and Public Health

Experimental Parasitology

Efficacy of protease inhibitor from marine Streptomyces sp. VITBVK2 against Leishmania donovani – An in vitro study

The present study was focused on screening and characterization of tyrosinase enzyme produced by marine actinobacteria and its application in phenolic compounds removal from aqueous solution. A total of 20 strains were isolated from marine sediment sample and screened for tyrosinase production by using skimmed milk agar medium. Among 20 isolates, two isolates LK-4 and LK-20 showed zone of hydrolysis and these were taken for secondary screening by using tyrosine agar medium. Based on the result of secondary screening LK-4 was selected for further analysis, such as tyrosinase assay, protein content and specific activity of the enzyme. The tyrosinase enzyme was produced in a SS medium and was partially purified by ammonium sulfate precipitation, dialysis and SDS PAGE. The isolate (LK-4) was identified as Streptomyces espinosus using 16S rRNA gene sequencing and named as "Streptomyces espinosus strain LK4 (KF806735)". The tyrosinase enzyme was immobilized in sodium alginate which was applied to remove phenolic compounds from water. The enzyme efficiently removed the phenolic compounds from aqueous solution within few hours which indicated that tyrosinase enzyme produced by Streptomyces espinosus strain LK-4 can be potently used for the removal of phenol and phenolic compounds from wastewater in industries. © 2014 Higher Education Press and Springer-Verlag Berlin Heidelberg.

Frontiers in Biology

Isolation and characterization of tyrosinase produced by marine actinobacteria and its application in the removal of phenol from aqueous environment

Objective: To investigate the antibacterial activity of marine actinobacteria against Multidrug resistance Staphylococcus aureus (MDRSA). Methods: Fifty one actinobacterial strains were isolated from salt pans soil, costal area in Kothapattanam, Ongole, Andhra Pradesh. Primary screening was done using cross-streak method against MDRSA. The bioactive compounds are extracted from efficient actinobacteria using solvent extraction. The antimicrobial activity of crude and solvent extracts was performed using Kirby-Bauer method. MIC for ethyl acetate extract was determined by modified agar well diffusion method. The potent actinobacteria are identified using Nonomura key, Shirling and Gottlieb 1966 with Bergey's manual of Determinative Bacteriology. Results: Among the fifty one isolates screened for antibacterial activity, SRB25 were found efficient against MDRSA. The ethyl acetate extracts showed high inhibition against test organism. MIC test was performed with the ethyl acetate extract against MDRSA and found to be 1000μg/ml. The isolated actinobacteria are identified as Streptomyces sp with the help of Nonomura key. Conclusion: The current investigation reveals that the marine actinobacteria from salt pan environment can be able to produce new drug molecules against drug resistant microorganisms. © 2012 Asian Pacific Tropical Biomedical Magazine.

Asian Pacific Journal of Tropical Biomedicine

In-vitro antimicrobial activity of marine actinobacteria against multidrug resistance Staphylococcus aureus

Journal of Applied Pharmaceutical Science

Distribution and antimicrobial activity of Thai marine actinomycetes

Introduction to GIS Programming and Fundamentals with Python and ArcGIS®

Python Language Control Structure, File Input/Output, and Exception Handling

Characterisation and identification of antibacterial compound from marine actinobacteria: In vitro and in silico analysis

Journal	Data powered by TypesetJournal of Infection and Public Health
Publisher	Data powered by TypesetElsevier BV
ISSN	1876-0341
Open Access	Yes