In India, there are two types of medical treatment systems using various drugs, namely English medical treatment systems and Ayurveda, Unani and Siddha treatment system (AUSTS). The experiments for medicinal drugs were carried out with the help of biomedical optical spectroscopy techniques such as ultraviolet/visible, Fourier transform infrared and Fourier transform Raman. The spectral analysis indicated that the specific functional groups of the drug materials have almost the same chemical characteristics. The following functional groups are present N=C=O, -N=C=S,-N=C=N-, -N3, O=C-C, O=C-N, C=C-N, C=C-C and CH 3, CH 2 and CH. After two dosages of the above drugs, the human body tissue sample were taken for test using Fourier transform infrared spectroscopy and it was found that the major chemical compounds are present and remaining functional groups are eliminated. © 2014 King Fahd University of Petroleum and Minerals.