This study described the removal of Ag(I) from wastewater using macrofungus Pleurotus platypus in a packed bed column. The effect of operating parameters such as bed height, flow rate and initial metal concentration were investigated on removal of Ag(I) under optimized condition. The breakthrough curve profile signified that the breakthrough time and exhaustion time increased with an increase in bed height and a decrease in flow rate and initial metal concentration. The data were supported by 3D mesh diagrams and perturbation plots obtained from 2D factorial design. The column data were analysed using four models viz., BDST model, Emperical model, Thomas model and Yan model. The BDST model was found to be the best to fit the breakthrough curves at experimental conditions. The column was regenerated using 0.01 M HCl solution and sorption-desorption studies were carried out for three cycles. The obtained results implied that P. platypus may serve as suitable adsorbent material for the removal of Ag(I) from wastewater.