Transesterification of Waste Cotton Cooking Oil was reported. Different catalysts were studied. A maximum yield of 92% was reported for calcined eggshells catalyst. KOH and pistachio shell reported 91% and 84% respectively. The temperature, reaction time and catalyst loading were optimized to be 60°C, 60 min and 3% (wt.%) respectively for egg shell and pistachio shells. The methanol to oil molar ratio suitable molar for transesterification catalyzed by calcined eggshell was 9/1 and 12/1 for pistachio shell. By using low cost catalysts from waste resources such as eggshell and pistachio shell for biodiesel from waste cotton cooking oil gives higher yield of production. It seems to be relatively easy to separate up to higher production of biodiesel, time saving, energy efficient and eco-friendly. © 2016 IEEE.