In Diabetes, self-monitoring of blood glucose is crucial for effective treatment since it can help to identify and prevent unwanted episodes of hypoglycemia and hyperglycemia. Aim of the present work is to design and develop a non invasive gluco-meter using near infrared LED (940 nm). Three different designs of probes (arm, finger and ear lobe) were fabricated to measures in-vivo blood glucose. In addition, we also carried out experiments to study the interaction with glucose molecules in solution under plane polarized light. The polarizers were used at three different angles(450, 900 and 1800) to know the behavior of glucose interaction with polarized light of 640 nm and 740 nm with varying concentration of glucose in the solution. The polarization results showed that at 1800 the glucose has maximum polarization (1.12) and at 900 and 450 it has comparatively less polarization values 0.9 and 0.35 respectively. The polarization results helped us to design the probes. Therefore, we placed photodiodes at three angles (450, 900 and 1800). The results as expected were better at 1800 by showing maximum variation in photodiode voltage output. The results were in the correlation of the polarization studies. A comparative study of three measurement sites has also been carried out. The primary result of current sensor system is promising and with further improvement a robust system for glucose sensing can be realized. © 2015 The Authors.