The screening of novel bacterial colonies from soil samples collected from various places was done followed by the plate screening using well diffusion method in casein skim milk agar plate. The desired bacterial colonies from each sample were subjected to bacterial growth curve study in order to observe the exponential phase of each colony. These bacterial colonies were then observed for highest protease activity by Folin Lowry's assay. The best bacterial colony was from the soil sample of milk packet dumping area followed by paper waste dumping area, cattle farm area and the least was observed in the soil sample from VIT lakeside. The best colony was chosen for further physical and chemical characterization which confirmed it to be a Bacillus strain. The optimization of pH showed the highest activity in alkaline conditions pH (at 9)which confirmed it to be alkaline protease. The statistical optimization for physical and nutritional parameters was done using Taguchi's orthogonal array method. Signal to noise ratio analysis from Taguchi's loss of function was done and the formula "larger is better" was used. From the main effects plot for SN ratio it was interpreted that the optimized pH for the bacillus is 9, optimized temperature is 45°C, optimized agitation is 100rpm, optimized NaCl is 0.6 g, and optimized peptone is 0.4g.