Over the past decades, numerous materials have emerged as promising amenities for the fabrication of novel membranes. The current study gives insight into a modest and effective method to fabricate a crosslinked poly-vinylidene fluoride-co-hexafluoropropylene membrane with better mechanical properties and permeability for desalination. Poly-vinylidene fluoride-co-hexafluoropropylene membrane was grafted with crosslinked collagen to enhance direct contact membrane distillation used for desalination. Stiffness, rigidity and mechanical properties of the membrane were intensified by incorporating collagen (extracted from eggshells) into the membrane matrix, with glutaraldehyde crosslinkers. Furthermore, to improve water vapor diffusion, immobilized carbon nanofibers (CNF) were integrated in the membrane, casted via phase inversion technique with an optimized controlled approach. The permeate flux of CNF incorporated membrane was as high as 8 LMH, 18% higher than the unmodified poly-vinylidene fluoride-co-hexafluoropropylene membrane at 60 °C, besides minimal salt leakage. The properties of the modified membrane were characterized from its contact angle, morphological structure, surface roughness, dynamic mechanical properties, and water flux. The overall performance of the modified membranes was better than the virgin membranes. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 48021. © 2019 Wiley Periodicals, Inc.