Ipomoea batatas popularly known as the sweet potato is a dicotyledonous plant that belongs to the Convolvulaceaefamily. Sweet potato tuber is a widely used source of nutrients. As not much work has been done on leaves, this study was undertaken to explore phytochemical, antioxidant, antimicrobial and anticancer activity of sweet potato leaves through different screening tests. Antimicrobial activities were studied by agar well diffusion method against nine clinical pathogens. The total antioxidant capacity was evaluated by the DPPH assay and followed by cytotoxicity activity on humanosteosarcoma (MG 63) cell line, using methanol and hexane extract as well as Thin Layer Chromatography (TLC) was performed for the separation of different components of the extract. Volatile extract of Sweet potato leaves were also interpreted using Gas Chromatography and Mass spectrometry. Both the extract showed good antimicrobial and antioxidant activity, whereas both methanol and hexane extract showed moderate anticancer activity. Sweet potato leaves are suitable for further clinical approach in near future. © RJPT All right reserved.