One of the most deliberate aggravation of ulcerative colitis commences to the development of colorectal cancer (CRC) owing to its high prevalence and mortality rates. The aim of our present study is to comprehensively explore the efficacy of the use of biogenic silver nanoparticles (AgNPs) cytotoxicity in primary colon cells and colorectal malignant cell line (HT-29). In addition, induction of apoptotic cell death by the influence of AgNPs was evaluated on HT-29 and primary cells in a dose-dependent manner. Formerly, the AgNPs were synthesised using Couroupita guianensis and its characterization was accomplished. We detail the execution of antiproliferative effect of biogenic AgNPs at Ic50 of 20μg/ml on human colorectal malignant cell line HT-29 whereas the primary colon cells proliferation was inhibited at IC50 of 40μg/ml at 24 h incubation. Collectively the results intended that AgNPs expend their anti-proliferative activity towards HT-29 controlling cell cycle phases by inducing apoptosis.