In the current scenario, medicinal plant research focuses on developing cancer-specific chemotherapeutic drugs which simultaneously render protection to normal cells. Hence, our study aimed to investigate the differential anti-neoplastic potentials of Oroxylum indicum ethyl acetate extracts (hot-EHO and cold-ECO). Cancer-cell-specific cytotoxicity of extracts was studied on MDA-MB-231 (cancer cells) and WRL-68 (normal cells). ECO showed significantly (p<0.05) better cytotoxicity in MDA-MB-231 cells (IC50 = 95.15±2.41 jag mL1) than in WRL-68 cells (IC50 = 203.54±2.96 jag mL1), thus substantiating cancer-cell-specific cytotoxicity. The mechanism behind cytotoxicity was further corroborated through apoptosis induction in MDA-MB-231 and MCF-7 cells. ECO has exhibited dose-dependent apoptotic induction in both the cells. ECO also showed evident anti-metastatic potential by significantly (p<0.05) inhibiting migration of metastatic breast cancer cells (MDA-MB-231). Over all, ECO exhibited efficient anti-breast cancer potentials and thus could be considered as a source harboring phytocomponents to possibly treat malignant breast cancer. © 2013 Asian Network for Scientific Information.