A major trend in medicine development today is the design of suitable vehicles for controlled release of drug in patients. Delivery vehicles for therapeutics must be biocompatible and benign thin polymer films have also been employed for colonic drug delivery. High molecular weight Chitosan based microspheres were prepared in order to deliver mebendazole as a model drug specifically into the colon. Microspheres were prepared by an emulsion and crosslinking method. The prepared microspheres are characterized by FTIR, SEM, and XRD, swelling studies investigated for controlled release of mebendazole as a model drug. The in vitro releases in pH 7.4 are reported. The results of this study suggest that the microspheres could be potentially useful for colon drug delivery of mebendazole over more than 12 hours.