There are many chronic lung diseases that occur in humans. In which some are diagnosed and some are yet under research. The main objective of the paper is to diagnose the condition that causes the chronic diseases by designing the underlying mechanisms of the lung. This project associates the changes in lung expression, especially for signaling pathway, through both clinical investigation in patients and the use of animal models, like neonatal exposure to hyperoxia in rodents. To achieve the aim, the simulation part is done using COMSOL Multiphysics. The accuracy and efficiency with resolution of the model can be achieved by COMSOL Multiphysics. This software opens a wide area to prove the mechanical properties and micro fluidic flow properties from micro level to nano level extend. The design can be incorporated with micro-pump, micro-mixer and gel electrophoresis component into a single model. The optimized design parameters and working principle offer an efficient and portable disease diagnostic system. Some patients may have a wide variety of diseases and they need conventional testing on the stage of disease which they undergo. This can be diagnosed by the proposed non-invasive technique. Beside this, the system can replace the animal testing model. © 2018 IEEE.