Vast growth has been noticed in the current years toward the use of fibres and clothing in the sportswear garment sector. Comfort properties of sports garments has been considered as one of the main function in developing the sports garment Lot of research work and ideas are taken care by garment manufacturers in making sportswear garments. Many more development in fabrics used for making sports garments especially for athletics were taken place in recent years. Mostly knitted fabrics are mainly used to make sportswear garments, which throws more scope of research and development in developing new knitted fabric structure with related high performance fibres New development of knitted fabric using the interlock construction leads to more space of developing knitted fabric with high comfort properties. Interlock fabrics made with separate kind of yarn in face, back and inlay with various structures enhances to produce knitted fabric with more comfort properties in sportswear. Knitted fabric is produced using different face, back and in lay yarn using interlock weft knitted structure has been carried out in this work and also their properties were studied thoroughly. To achieve more comfort and essential properties in weft knitted interlock fabric to suit sportswear, the fabric in this research work has been produced using yarn made up of cotton, polyester and viscose. Various Knitted fabrics has been produced by altering fabric structure, yarn linear density (Yarn count). The properties of fabric have been analyzed with respect to its air permeability, moisture management and thermal conductivity. © BEIESP.