Diabetic retinopathy is one of the important reasons for vision loss that arises due to diabetes. Non-proliferative diabetic retinopathy is the preliminary stage of diabetic retinopathy disease which shows initial symptoms in the retina of human eye. In this paper, Microaneurysms are detected using different preprocessing steps and mathematical morphology techniques. Histogram stretching and CLAHE are used to give uniform illumination to the poor quality images. Morphology techniques are used remove the optic disc which may be misclassified as noise and to provide better visibility of Microaneurysms. This method provides us with better result inorder to identify Microaneurysms clearly without any misclassification. © International Science Press.