A novel and highly sensitive probe for the detection of organophosphorus compounds (OPs) using acetylcholinesterase (AChE) and acetylthiocholine (ATCh) during the modulated synthesis of silver nanoparticles.

Amitava Mukherjee

Centre for Nanobiotechnology

Vellore Campus

Chandra Sekaran N

Nanda Kumar D

Rajeshwari A

Alex S.A

Sahu M

Raichur A.M

Vellore Institute of Technology (VIT) is a private university located in&nbsp;Tamil Nadu, India. Founded in 1984, as Vellore Engineering College, the institution offers 20 undergraduate, 34 postgraduate, four integrated and four research programs. It has campuses in Vellore, Amravati, Bhopal and Chennai.

VIT is one of the top ranked private universities in India according to NIRF, THE and QS Rankings.&nbsp;Govt. of India has recognized&nbsp;VIT, Vellore as an&nbsp;Institution of Eminence. This has allowed VIT to take independent quality initiatives and move up in world ranking.

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VIT University

RSC Advances

Herein, we report the enzyme-mediated synthesis of bimetallic nanostructure consisting of gold nanoclusters shell- silver nanoparticles core (AuNCs-AgNPs) for catalytic reduction applications. For the synthesis of core AgNPs, thiocholine (TCh), an enzymatic product from the acetylcholinesterase (AChE), and acetylthiocholine (ATCh) interaction was used as a stabilizing agent. The AuNCs-AgNPs was prepared using TCh as a reducing as well as stabilizing agent. The synthesized AuNCs-AgNPs were characterized with help of UV–Vis spectroscopy, dynamic light scattering (DLS) analyses and High-Resolution Transmission Electron Microscopy (HR-TEM). The HR-TEM micrographs confirmed that the formation and arrangement of AuNCs on the core AgNPs, resulting in AuNCs-AgNP bimetallic structures. The catalytic reduction activity of thus prepared AgNPs and AuNCs-AgNPs was investigated against methyl orange (MO) and potassium ferricyanide K3[Fe(CN)6] in the presence of sodium borohydride (NaBH4). The rate constants (k), for MO and K3[Fe(CN)6] catalytic reduction in the presence of AuNCs -AgNPs were measured to be 2.3 × 10− 2 s− 1 and 1.219 × 10− 1 s− 1 respectively. © 2020 Elsevier B.V.

Journal of Molecular Liquids

Novel enzymatic synthesis of core/shell AgNP/AuNC bimetallic nanostructure and its catalytic applications

Biocatalyzed gold nanoparticle synthesis for the in situ detection of horseradish peroxidase inhibitor (phenylhydrazine) has been demonstrated.

New Journal of Chemistry

A novel enzyme-mediated gold nanoparticle synthesis and its application for in situ detection of horseradish peroxidase inhibitor phenylhydrazine

We report an enzyme-mediated biosensor with the immobilization of silver nanoparticles (AgNPs) for the detection of organophosphorus (OP) pesticides.

Acetylcholinesterase (AChE)-mediated immobilization of silver nanoparticles for the detection of organophosphorus pesticides

A novel fluorimetric detection method for Hg2+ and Pb2+ based on in situ formation of AgNPs, where thiocholine (TCh), a product obtained by the hydrolysis of acetylcholine (ACh) by acetylcholinesterase (AChE), can act as a stabilizing agent.

Spectrofluorimetric determination of Hg2+ and Pb2+ using acetylcholinesterase (AChE)-based formation of silver nanoparticles

Journal of Agricultural and Food Chemistry

Silver Nanoparticle-Based Chemiluminescent Sensor Array for Pesticide Discrimination

Sensors

Nanomaterials-Based Optical Techniques for the Detection of Acetylcholinesterase and Pesticides

Sensors and Actuators B: Chemical

Unmodified silver nanoparticles for rapid analysis of the organophosphorus pesticide, dipterex, often found in different waters

The Analyst

Thiocholine mediated stabilization of in situ produced CdS quantum dots: application for the detection of acetylcholinesterase activity and inhibitors

A highly sensitive sensing system based on photoluminescent quantum dots for highly toxic organophosphorus compounds

Developing acetylcholinesterase-based inhibition assay by modulated synthesis of silver nanoparticles: applications for sensing of organophosphorus pesticides

Journal	Data powered by TypesetRSC Advances
Publisher	Data powered by TypesetRoyal Society of Chemistry (RSC)
ISSN	20462069
Open Access	0