A new class of imaging probe, a fluorogenic version of 1, 3-dipolar cycloaddition of azides and alkynes has been developed. 3-azidocoumarin scaffolds were selectively immobilized on the DBCO modified bead surface via SPAAC and provide direct and strong fluorescence in fluorescence microscopy. This developed click-on beads could be applied to label various biomolecules, such as nucleic acids, proteins and other molecules. To this end, 5′(7-hydroxy 3-azido coumarin) labelled DNA primer also displayed strong fluorescence upon successful immobilization on the bead surface. © 2015 Elsevier Ltd.

Priyankar Paira

Department of Chemistry

School of Advanced Sciences

Vellore Campus

Bharathi M.V

Chhabra M

Vellore Institute of Technology (VIT) is a private university located in&nbsp;Tamil Nadu, India. Founded in 1984, as Vellore Engineering College, the institution offers 20 undergraduate, 34 postgraduate, four integrated and four research programs. It has campuses in Vellore, Amravati, Bhopal and Chennai.

VIT is one of the top ranked private universities in India according to NIRF, THE and QS Rankings.&nbsp;Govt. of India has recognized&nbsp;VIT, Vellore as an&nbsp;Institution of Eminence. This has allowed VIT to take independent quality initiatives and move up in world ranking.

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VIT University

Bioorganic & Medicinal Chemistry Letters

Here, surface immobilization of DNA conjugates via SPAAC for cancer theranostic applications was reported.

New Journal of Chemistry

Surface immobilization of biotin-DNA conjugates on polystyrene beads via SPAAC for biological interaction and cancer theranostic applications

Recently a choice of fluorescent bioimaging probes have been developed as medical diagnostic tools. Herein, we have introduced a series of coumarin-based target specific probes for cancer theranostic application which play a dual role in the field of both diagnosis and therapy. A fluorogenic version of 1,3-dipolar cycloaddition between azides and alkynes (DBCO) has been introduced to develop the triazolylcoumarin based fluorescent scaffolds. These scaffolds were screened for their anticancer activity against breast cancer (MCF7) and human epitheloid cervix carcinoma (HeLa) cell line. It was established that triazolylcoumarins (5c and 5d) are having electronegative substitution in the benzene ring displayed most effective anticancer profile in both the cell lines. Compounds 5a and 5d exhibited maximum quantum yield and strong cellular uptake in the MCF-7 cell line. © 2016 Elsevier Ltd

Synthesis and cytotoxicity study of novel 3-(triazolyl)coumarins based fluorescent scaffolds

Scientific Reports

Near-infrared fluorescence imaging of cancer cells and tumors through specific biosynthesis of silver nanoclusters

Carbon Nanoparticle-based Fluorescent Bioimaging Probes

Chemistry - A European Journal

A Non-Fluorinated Monobenzocyclooctyne for Rapid Copper-Free Click Reactions

Chemical Communications

Immobilisation of quantum dots by bio-orthogonal PCR amplification and labelling for direct gene detection and quantitation

Accounts of Chemical Research

Biomedical Applications of Tetrazine Cycloadditions

Development of surface immobilized 3-azidocoumarin-based fluorogenic probe via strain promoted click chemistry

Journal	Data powered by TypesetBioorganic & Medicinal Chemistry Letters
Publisher	Data powered by TypesetElsevier BV
ISSN	0960-894X
Open Access	0