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Direct organogenesis from ray and disc florets of a newly evolved chlorophyll variegated chrysanthemum (chrysanthemum morifolium)
S.K. Datta, , M. Saxena
Published in
2001
Volume: 71
   
Issue: 10
Pages: 655 - 657
Abstract
Recently developed cultivar 'Kargil 99' is a small flowered, spoon-shaped, single whorl purplish mouve ray florest with yellow disc and snow white variegation on leaves. Both ray and disc florets from half-open flower heads were cultured on Murashige and Skoog's (MS) medium supplemented with different concentrations of BA and NAA. Direct shoot organogenesis was observed within 20-25 days. Disc florets were more organogenic than ray florets. The highest (75%) organogenic response with 11 shoots/responding explants was obtained from disc florets in 1 mg/litre NAA and 5.0 mg/litre BA treatment. Shoots were rooted on 0.2 mg/litre NAA containing half-strength MS medium. Survival of plantlets was 100% upon transfer to the field conditions where they flowered true-to-type.
About the journal
JournalIndian Journal of Agricultural Sciences
ISSN00195022