The discussion on the application of natural bio hybrid organic-inorganic fillers (crab shell particles) for coating application forms the focus of this work. The utilization of shells of marine organism is growing enormously in view of its mechanical and thermal properties. However, the application of proteinized crab shell particles (CSP) or deproteinized crab shell particles (DCSP) for coating field remains a question mark. Deproteinization is done by removing the organic matrix from the crab shell particles using the deproteinization/depolymerization method. Washburn capillary test, adsorption and hydro-stability of crab shell were conducted for studying the surface property of particles in hydro environment. The maximum adsorption capacity was observed for CSP in contrast to hydrophobic DCSP. Stability test had shown the dissolution of CSP. Thermogravimetric analyzer and differential scanning calorimeter results have shown the thermal instability of DCSP on the removal of organic matrix from the crab shell particles. Characterization was done extensively using Fourier transform infrared spectroscopy, X-ray diffractometer and scanning electron microscope. © 2016 Elsevier Ltd.