Medicinal plant products are the natural products which have been very useful for human to cure various ailments and as an alternative medicine for conventional therapy. However, bacteria in natural environments are mainly exist in biofilm formation and are more susceptible to cause severe infections than the planktonic counterparts. Biofilm is associated with impaired epithelization and granulation tissue formation and also promotes a low-grade inflammatory response that interferes with wound healing. Since the infection caused by biofilm is often very difficult to treat, there is a need to find a new active anti-biofilm agent. In recent past, interest in the therapeutic and nutritional properties of various medicinal plants and its natural phytochemical compounds which have established for their anti-biofilm activities has been increased gradually. In this review, we have described various aerial parts of medicinal plants which have anti-biofilm effect which was evaluated against biofilm producing different bacterial pathogens and antimicrobial agents which are responsible to cure wound healing.Keywords: Medicinal plants, Phytochemical, Anti-biofilm activity.