A 3D numerical model is developed to analyze the force distribution over a single BV2 cell, when the cell is under biomechanical stress within a microfluidic platform. The simulation can predict the shear stress and deformation, respectively, over the cell surface and nucleus under hydrodynamic forces. The two main factors for cellular deformation-pressure and viscous forces were studies. It is found that, in the front-half of the cell facing the flow, pressure forces are greater than the viscous forces. Finally, the cellular deformation and shear stress changes are studied at two conditions; when the BV2 cell is completely- and partly- attached to the substrate. It is observed that the shear stress over the cell increases, when the contact of the cell with the substrate decreases.