Objective: Catheter associated urinary tract infection (CAUTI) is considered as one of the prevalent nosocomial infection, where the severity directly correlates with the period of catheterization. Strategies like improved biomaterials and anti-microbial coatings lack enough evidences to be considered as an effective scheme against CAUTI and retain the risk of developing resistance. Since most of the virulence factors in pathogens are under quorum sensing (QS) switch, QS antagonists are favored to be an effective policy against CAUTI. Hence, the aim of this study was to evaluate the anti-QS activity of sub-inhibitory concentrations (SIC) of selected antibiotics. Methods: Five different antibiotics; Nalidixic acid (NAL), Ampicillin (AMP), Amoxicillin (AMX), Cefuroxime (CXM), Co-trimoxazole (COT) were selected and Azithromycin (AZM) was used as the positive control. The SIC were determined against P. aeruginosa PAO1 and 2 clinical isolates of Pseudomonas sp. (PA-1 and PA-2). All the test compounds were quantified for the reduction in expression of the QS regulated behaviors. Results: All the antibiotics under investigation exhibited reduction in the production of QS controlled factors in the test organisms. However, CXM at 20 μg/mL, 36 μg/mL and 32 μg/mL displayed significant reduction in the quorum regulated genes of PAO1, PA-1 and PA-2 respectively in comparison with the positive control AZM. While the other test compounds failed to generate comparable results. Conclusion: It has been surmised that cephalosporin antibiotic-CXM effectively subdued QS regulated factors and could be explored as a superior prospect against CAUTI, either as a coating or as a wash solution.