N -nitrosodimethylamine (NDMA) is known to be highly carcinogenic and is present in drinking water, wastewater, and a variety of foods. Because of its presence in chloraminated water at nanogram per liter concentrations, NDMA has become an emerging issue for reclaimed water which may be used for aquifer recharge or irrigation. This research investigated the fate of NDMA in two soil column systems used to simulate subsurface transport. One column system was operated under aerobic conditions with increasing primary substrate concentration where the biodegradable organic carbon (BDOC) in reclaimed water was used as the primary substrate. The reclaimed water content in the influent was increased from 0 to 25% in the column to increase the BDOC concentration. Negligible NDMA removal was observed at 0% reclaimed water and increasing the primary substrate in the influent resulted in NDMA removal suggesting that biodegradation of NDMA might be a cometabolic process. The effects of redox conditions on NDMA fate was studied by operating a second column system with 100% reclaimed water under anoxic conditions and then changing the conditions to aerobic. It was observed that NDMA removal was similar under both aerobic and anoxic condition, however, much lower effluent concentrations were observed under aerobic conditions. Under anoxic condition, a normalized mass removal rate of 254 ng NDMA/mg DOC was observed which increased to 273-ng NDMA/mg DOC under aerobic conditions. The majority of NDMA and substrate removal occurred in the first of three columns in series column under both aerobic and anoxic conditions. Normalized mass removal rates of NDMA after the first, second, and third columns were 372, 30, and 20 ng NDMA/mg DOC, respectively. Since the majority of dissolved organic carbon was also removed in the first column, NDMA biodegradation was consistent with cometabolic activity. Batch tests verified the biodegradation removal potential of NDMA. Addition of a methylotrophic substrate, methanol and an aromatic substrate, toluene, did not increase NDMA removal. © 2010 ASCE.