Jamuna K

Electrical

School of Electrical Engineering

Chennai Campus

Ponrasu T

Mathew A

Madhukumar K.N

Ganeshkumar M

Iyappan K

Suguna L.

Vellore Institute of Technology (VIT) is a private university located in&nbsp;Tamil Nadu, India. Founded in 1984, as Vellore Engineering College, the institution offers 20 undergraduate, 34 postgraduate, four integrated and four research programs. It has campuses in Vellore, Amravati, Bhopal and Chennai.

VIT is one of the top ranked private universities in India according to NIRF, THE and QS Rankings.&nbsp;Govt. of India has recognized&nbsp;VIT, Vellore as an&nbsp;Institution of Eminence. This has allowed VIT to take independent quality initiatives and move up in world ranking.

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VIT University

Amino Acids

The aim of this article was to develop silk protein (SF) and l-proline (LP) loaded chitosan-(CS) based hydrogels via physical cross linking for tissue engineering and wound healing applications. Silk fibroin, a biodegradable and biocompatible protein, and l-proline, an important imino acid that is required for collagen synthesis, were added to chitosan to improve the wound healing properties of the hydrogel. Characterization of these hydrogels revealed that CS/SF/LP hydrogels were blended properly and LP incorporated hydrogels showed excellent thermal stability and good surface morphology. Swelling study showed the water holding efficiency of the hydrogels to provide enough moisture at the wound surface. In vitro biodegradation results demonstrated that the hydrogels had good degradation rate in PBS with lysozyme. LP loaded hydrogels showed approximately a twofold increase in antioxidant activity. In vitro cytocompatibility studies using NIH 3T3 L1 cells showed increased cell viability (p < 0.01), migration, proliferation and wound healing activity (p < 0.001) in LP loaded hydrogels compared to CS and CS/SF hydrogels. Cell adhesion on SF and LP hydrogels were observed using SEM and compared to CS hydrogel. LP incorporation showed 74-78% of wound closure compared to 35% for CS/SF and 3% for CS hydrogels at 48 h. These results suggest that incorporation of LP can significantly accelerate wound healing process compared to pure CS and SF-loaded CS hydrogels. Hence, CS/LP hydrogels could be a potential wound dressing material for the enhanced wound tissue regeneration and repair. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1401–1408, 2017. © 2016 Wiley Periodicals, Inc.

Journal of Biomedical Materials Research - Part B Applied Biomaterials

Biomimetic hydrogel loaded with silk and l-proline for tissue engineering and wound healing applications

We have developed L-glutamic acid (LG) loaded chitosan (CS) hydrogels to treat diabetic wounds. Although literature reports wound healing effects of poly(glutamic acid)-based materials, there are no studies on the potential of L-glutamic acid in treating diabetic wounds. As LG is a direct precursor for proline synthesis, which is crucial for collagen synthesis, we have prepared CS + LG hydrogels to accelerate diabetic wound healing. Physiochemical properties of the CS + LG hydrogels showed good swelling, thermal stability, smooth surface morphology, and controlled biodegradation. The addition of LG to CS hydrogels did not alter their biocompatibility significantly. CS + LG hydrogel treatment showed rapid wound contraction compared to control and chitosan hydrogel. Period of epithelialization is significantly reduced in CS + LG hydrogel treated wounds (16 days) compared to CS hydrogel (20 days), and control (26 days). Collagen synthesis and crosslinking are also significantly improved in CS + LG hydrogel treated diabetic rats. Histopathology and immunohistochemistry results revealed that the CS + LG hydrogel dressing accelerated vascularization and macrophage recruitment to enhance diabetic wound healing. These results demonstrate that incorporation of LG can improve collagen deposition, and vascularization, and aid in faster tissue regeneration. Therefore, CS + LG hydrogels could be an effective wound dressing used to treat diabetic wounds. © 2017 The Author(s).

Fulltext

Scientific Reports

Accelerated Healing of Diabetic Wounds Treated with L-Glutamic acid Loaded Hydrogels Through Enhanced Collagen Deposition and Angiogenesis: An in Vivo Study

Composite 3D scaffolds using a natural, carbohydrate polymer, isabgol (Isab) and silk fibroin (SF) were prepared by a freeze drying method. 2 wt% solutions of both Isab and SF were blended in four different ratios to obtain the 3D scaffolds. ATR-FTIR results showed the presence of amide bonds and β sheet conformation in Isab/SF scaffolds. SEM micrographs showed the fibrous foam like architecture in the Isab/SF blend. Scaffolds showed sufficient porosity and absorbed higher amounts of PBS solution, enhancing the gas and nutrient supply to the cells. Subsequently, scaffolds showed significant in vitro biodegradation. In vitro cytocompatibility test in NIH 3T3 fibroblast cells showed better viability, proliferation and cell attachment for the Isab/SF 75/25 scaffolds compared to the control. Among all the scaffolds, Isab/SF 75/25 showed a faster wound contraction rate (p < 0.001) and reduced period of epithelialization (16 days) compared to the control (23 days). Shrinkage temperature and collagen content was also increased significantly (p < 0.001) in Isab/SF 75/25 scaffold treated rats compared to the control. Histopathology results strongly demonstrate higher cellular infiltration, increased fibroblasts, dense collagen fibers, and neovascularization in Isab/SF 75/25 treated rats, compared to other treatments. Overall, these results strongly authenticate that the Isab/SF 75/25 3D composite scaffolds enhanced the fluid uptake ability with enough fibroblast attachment and good viability. Also, it accelerated tissue regeneration during wound healing in rats without addition of any bioactive molecules. © 2016 The Royal Society of Chemistry.

RSC Advances

Isabgol-silk fibroin 3D composite scaffolds as an effective dermal substitute for cutaneous wound healing in rats

International Wound Journal

Efficacy ofAnnona squamosaon wound healing in streptozotocin-induced diabetic rats

Wound-healing potential of an ethanol extract ofCarica papaya(Caricaceae) seeds

American Journal of Physiology-Regulatory, Integrative and Comparative Physiology

Arginine plus proline supplementation elicits metabolic adaptation that favors wound healing in diabetic rats

Wound Repair and Regeneration

Extracellular matrix-derived tripeptide proline-glycine-proline inhibits keratinocyte proliferation and migration

Phytotherapy Research

Wound-healing Properties of the Oils of Vitis vinifera and Vaccinium macrocarpon

Efficacy of l-proline administration on the early responses during cutaneous wound healing in rats

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Publisher	Data powered by TypesetSpringer Science and Business Media LLC
ISSN	0939-4451
Open Access	0