Big bio business is going on enzymes because of wide variety of industries using commercial biocatalysts, in addition to the feed industry. Three-quarters of the market is for enzymes involved in the hydrolysis of natural polymers. Food processing enzymes including pectinases in juice industries account for about 45 percent of enzyme usage. Mostly in primary cell walls of plants a complex set of polyssachrides, pectin is present. It is also present in middle lamella where it help to bind cells together. Pectin’s chemical composition, amount and structure will be different among variety of plants and over time. In our study we carried out the studies to determine pectinolytic activity from Bacillus VIT sun-2 and in combination with xylanase and cellulase to improve yield and clarification of culinary juices. © 2015, Sphinx Knowledge House. All rights reserved.