The current research was undertaken to determine the potency of two distinct probiotics Biogut® Aqua (feed probiotic) and Bioclean® Aqua (water and soil probiotic) to induce immunity and protection faced due to Vibrio pathogen during cultivation of Mozambique tilapia under controlled laboratory trials. Assessment was carried out using immune gene expression and immunological assays. During the initial phase of treatment, nitro blue tetrazolium (NBT) assay, antiprotease assay and peroxidase assay displayed a convincing raise in serum activity of the probiotic managed feed when compared to the control groups. In addition, qPCR real time analysis displayed in which nutritional implementation with feed probiotic vitally increased the interpretation of immune related genes, particularly TNF and MCP gene expression which are involved in immune response. The results revealed that the probiotics Bioclean® Aqua and Biogut® Aqua have beneficial effects through efficiently enhancing immune system and protection in tilapia model facing Vibriosis. Hence it can be stated that the application of probiotics like Biogut® Aqua and Bioclean® Aqua in aquaculture environment may significantly reduce the antibiotic usage, thereby preventing the entry of these chemicals into the food chain. Additionally it was demonstrated that the ammonia level in water can be reduced significantly with the application of Bioclean® Aqua (water and soil probiotic). The toxicity studies proved that both the probiotic are non-toxic and safe for the use in aquaculture environment. © 2020 National Institute of Science Communication and Information Resources (NISCAIR). All rights reserved.