Nanoparticles could act as the efficient catalyst or the inhibitor towards the fibrillation pathway of biomolecules. In the present work, spectroscopic approaches including Thioflavin T fluorescence, Congo red dye absorbance, tryptophan fluorescence emission, three-dimensional fluorescence and circular dichroism spectra has been used to detect and evaluate the fibrillation induced conformational changes in HSA in the existence of CNTs. CR dye absorbance showed the red shift from 495 to 500 nm upon binding with fibrils. Similarly, ThT fluorescence emission upon the excitation at 450 nm showed the gradual increase in the intensity that conveyed the enhanced formation of fibrils in the presence of CNTs. Trp emission maxima obtained at 344 nm gradually decreased with no prominent shift in the fibrillar samples. Changes in the 3D spectral peaks 1 and 2 of native and fibrillar solutions suggested the alteration in the aromatic amino acid residues micro-environment. CD results showed increased percentage contents of beta-sheet structures in the fibrillar solutions interacted with CNTs. Hence, the research outcome of the study conveys a promoting role of surfactant detached SW and MWCNTs against HSA fibrillation in vitro. © 2016 Elsevier B.V. All rights reserved.