Encapsulation of the most potent combination of staphylokinase and Leucasaspera plant extract was done using chitosan nanoparticles. Chitosan is a biodegradable and non-toxic polysaccharide that can act as a drug carrier. Chitosan nanoparticles were prepared based on the ionic gelation of chitosan with tripolyphosphate(TPP) anions. The physicochemical properties of the nanoparticles were determined by atomic force microscopy (AFM), FTIR analysis and XRD. © 2015, Sphinx Knowledge House. All rights reserved.