Electrospun bioactive nanofibers have great potential as promising reinforcement fillers for next generation bone tissue engineering application. In the present study, electrospun nanofibrous membrane (ENM) prepared from blend of poly (vinyl) alcohol (PVA), nanohydroxyapatite (nano-HA) using fish bone, collagen type I (CT I) from fish bone, and graphene oxide (GO) by electrospinning for their prospective application in bone regeneration. The prepared nanofibrous membrane was characterized using physicochemical and mechanical properties. Scaffold prepared from PVA:nano-HA:CTI:GO possessed better physicochemical and mechanical properties. Invitro studies were carried out using human keratinocyte cell line (HaCaT) which proved the biocompatibility of the nanofibrous membrane and its more viable cells present in the nanofibrous membrane (PVA:nano-HA:CTI:GO). The article discusses in detail the applicability of these nanofibrous membrane in tissue engineering such as bone, cartilage, ligament, skin and as vehicle for the controlled delivery of drugs and future trends. © 2019 Elsevier B.V.

Sumathi V

Electrical

School of Electrical Engineering

Chennai Campus

Rethinam S

Wilson Aruni A

Munusamy C

Gobi N.

Vellore Institute of Technology (VIT) is a private university located in&nbsp;Tamil Nadu, India. Founded in 1984, as Vellore Engineering College, the institution offers 20 undergraduate, 34 postgraduate, four integrated and four research programs. It has campuses in Vellore, Amravati, Bhopal and Chennai.

VIT is one of the top ranked private universities in India according to NIRF, THE and QS Rankings.&nbsp;Govt. of India has recognized&nbsp;VIT, Vellore as an&nbsp;Institution of Eminence. This has allowed VIT to take independent quality initiatives and move up in world ranking.

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VIT University

Journal of Drug Delivery Science and Technology

The present study, COLL: AV: GPO bioactive scaffold prepared from collagen (COLL), Aloe vera (AV) and graphene oxide (GPO) for wound healing application. Bioactive scaffolds were examined by Fourier Transform Infrared Spectroscopy (FT-IR), Thermogravimetry Analysis (TGA) to understand the intermolecular interaction and their influence on the thermal property of the composite. Scanning Electron Microscope (SEM) image of the bioactive scaffold showed porous and smooth surface. Atomic Force Microscopy (AFM) observed fibrils with intermittent banding patterns. Bioactive scaffold possessed tensile strength (MPa), elongation at break (%), and water absorption properties (%). Bioactive scaffold exhibited good anti-bacterial activity against E.coli and S.aureus. MTT assay on the composites showed enhanced cell growth of human keratinocyte cell line (HaCaT) without exhibiting any toxicity. A higher rate of cell growth present in the COLL: AV: GPO bioactive scaffold compared to the other samples (COLL and COLL: AV) was observed. Acceleration of wound healing in COLL: AV: GPO bioactive scaffold treated rats were significantly in the in vivo experiments. These results suggested that the COLL: AV: GPO bioactive scaffold could be promising materials from wound healing applications. © 2019 Elsevier B.V.

Materials Chemistry and Physics

Enhanced tissue regeneration using an nano- bioactive scaffold- A novel perspective

The discipline of tissue engineering opens up the ways for repair and regenerate damaged organs and tissues. In the current work biomimetic nanofibrous scaffolds were fabricated by electrospinning. Poly-L-lactic acid (PLLA) was blended with collagen and gelatin to fabricate PLLLA-collagen and PLLA-gelatin fibrous scaffolds respectively. Pure PLLA and gelatin scaffolds served as controls. All the scaffolds displayed randomly oriented smooth fibers studied by SEM. Surface topography and roughness were studied by AFM and surface contact angle was also measured for all the fabricated scaffolds. Surface roughness was found to be higher in collagen and gelatin blended scaffolds in comparison to PLLA scaffold. Blending of collagen and gelatin reduced the surface hydrophobicity of the scaffolds. Human osteosarcoma cell lines MG-63 were cultured on all scaffolds up to 7 days and cell adhesion was studied through SEM and confocal microscopy. SEM and confocal results showed that gelatin blended PLLA scaffold showed better cell attachment and cell spreading. © 2016. All Rights Reserved.

Fulltext

Brazilian Archives of Biology and Technology

Nanofibrous Scaffolds for Tissue Engineering Applications

Preparation of absorbable surgical suture: Novel approach in biomedical application

The International Journal of Artificial Organs

Electrospun poly(vinyl) alcohol/collagen nanofibrous scaffold hybridized by graphene oxide for accelerated wound healing

American Journal of Materials Synthesis and Processing

Hydroxyapatite and Demineralized Bone Matrix from Marine Food Waste – A Possible Bone Implant

Progress in Materials Science

Mechanical properties of graphene and graphene-based nanocomposites

Enhanced bone regeneration using an electrospun nanofibrous membrane – A novel approach

Journal	Data powered by TypesetJournal of Drug Delivery Science and Technology
Publisher	Data powered by TypesetElsevier BV
ISSN	1773-2247
Open Access	0