We investigated the use of zinc imprinted of novel nanobiosorbent prepared from Candida rugosa to remove Zn(II) from aqueous solution. The nanobiosorbent was characterized by SEM, FTIR and XRD. Effects of various parameters including pH of the solution, adsorbent dosage, initial Zn(II) ion concentration and contact time on Zn(II) removal by the nanobiosorbents were investigated through batch process. Equilibrium data for Zn(II) removal was fitted to Langmuir isotherm model with an enhanced adsorption capacity of 275.48mg/g for zinc imprinted C. rugosa nanobiosorbent, compared to nonimprinted nanobiosorbent of 172.41 mg/g. Pseudo-second-order kinetic model was best fitted to predict the sorption kinetics for both the nanobiosorbents. AFM study revealed monolayer adsorption with thin film diffusion for Zn(II) removal. The antimicrobial activity of zinc imprinted nanobiosorbent was investigated against pathogenic yeasts viz. Candida albicans and Cryptococcus neoformans using agar well diffusion method. {\textcopyright} 2014 Korean Institute of Chemical Engineers, Seoul, Korea.