Amycolatopsis decaplanina JAR8 is rare actinomycetes which was isolated from agricultural field of Punjab, India. The 16S rRNA gene sequencing of strain JAR8 showed 98% similarity with Amycolatopsis decaplanina. The morphological, cultural and physiological characteristics were determined by International Streptomyces project by using various mediums. The bioactive metabolites produced by the strain were obtained from the optimized culture medium through solvent extraction method. The ethyl acetate extract obtained after extraction was characterized using UV, FTIR and GC-MS spectroscopic analysis. The minimum inhibitory concentration of active metabolite was found to be 60 μg/ml against Staphylococcus aureus. The present study deals with the exploration of rare actinomycetes which also produces various biological active compounds against infectious diseases. © 2014, Global Research Online. All rights reserved.