Abstract: The main objective of this study (ABST and MIC) is used to recognize the possible drug resistance in microorganisms like Escherichia coli, Staphylococcus aureus and to assure susceptibility to drug of choice for particular infections. Here we use various medicinal plant extracts like Basil leaves (Ocimum tenuiflorum), Aloe vera (Aloe barbadensis miller), karpuravalli (Plectranthus amboinicus), kuppaimani (Acalypha indica) to determine an antimicrobial activity. By using different concentrations of these extracts, we evaluate whether the bacteria are susceptible to these compounds or not. We use Gram positive and Gram-negative microorganisms for the evaluation of pharmacological examinations and furthermore we have used immobilization technique for long term preservation. This technique will be useful to keep the medicinal plant extracts in an active form for a longer period of time.