Aloe vera (Aloe barbadensis Miller) is a perennial succulent plant belonging to the Aloeaceae family and has been used for many centuries for its medicinal properties. Among the Aloe vera plant parts, gel is being widely studied for their biological properties. However, epidermis and flowers are neglected and are not probed for their importance. In this study, the methanolic extracts of Aloe vera gel, leaf and flower were evaluated for their anti-oxidant and anti-diabetic potential in vitro and other phytochemical constituents. We observed that the Aloe vera gel, leaf and flowers have shown significant anti-oxidant potential. When compared between leaf, gel and flower of Aloe vera, the leaf showed better antioxidant activity in ABTS, DPPH, H2O2 radical scavenging activities, Metal chelating activity and reducing power. However, the gel extract showed better anti-diabetic efficacy compared to the flower extract with respect to α-amylase, α-glucosidase, DPP-IV inhibition and anti-glycation activities. This study suggests that different parts of Aloe vera exhibit great potential for antioxidant and anti-diabetic activity and may be useful in new health-care food supplements or nutraceutical. © RJPT All right reserved.