The present work was to investigate the phytochemical analysis and to evaluate in vitro antioxidant, antibacterial and antibiofilm activities of methanol and petroleum ether leaf extracts of Aerva lanata. Dried leaves of Aerva lanata were extracted by methanol and petroleum ether solvent. The preliminary phytochemical analysis was performed on the leaf extracts of Aerva lanata using standard procedure. Antioxidant activity, total phenolic, and total flavonoid content of the leaf extracts of Aerva lanata were determined using spectrophotometric methods. In-vitro antibacterial and antifungal activities of leaf extracts of plant against the bacterial pathogens (E.coli, B.subtilis, P.vulgaris and S.aureus) and fungal pathogens (Aspergillus fumigatus and Aspergillus niger) was analysed through well diffusion and MIC methods and compared with the standard drugs. Antibiofilm activity was tested by crystal violet assay. The two extracts was further subjected to GC-MS analysis. The phytochemical analysis carried out for leaf extracts of plant showed the presence of several secondary metabolites. Methanol extract of leaf showed the highest phenolic and flavonoid content and high antioxidant activity. Biological activities of methanol leaf extract showed good inhibitory activity against the bacterial than fungal pathogens. The MIC was recorded in the range of 50-6.25μg/ml and 50-12.5μg/ml against the bacterial and fungal pathogens, respectively. The leaf extracts of Aerva lanata for antibiofilm activity against the bacterial pathogens were statistically significant. The leaf extracts of Aerva lanata on pharmacological activities like antibacterial, antioxidant, antibiofilm were explored. The pharmocogonostic activity of leaf extracts of Aerva lanta has research further. © 2016, Global Research Online. All Rights Reserved.