As a plausible source for the next generation heat transfer applications, Nanofluids have attracted many researchers. It became very prominent field in the past decade due to its major change in the physical and thermal properties in microscopic level compared to the same material in macroscopic level. Many experimental research and theoretical investigations have been carried out to study the thermal and physical properties of Nanofluids. Radiators are heat exchangers and used as cooling system in the internal combustion engines. To analyse the cooling performance of the car radiator an experimental setup has been devised. In this experimental study the mixture of ethylene glycol and water combination (40:60) with TiO2 Nanoparticles is used. The volume Concentration of 0.2 % is prepared by mixing TiO2 Nanoparticles with magnetic stirrer. Results of Thermal conductivity, heat transfer is obtained in the temperature range of 50°C to 80°C. Heat transfer is enhanced for the study with Nanofluids compared with the base fluid. © IAEME Publication.