Fluoroquinolone antibiotics are among the most potent second-line drugs used for treatment of multidrug-resistant tuberculosis and resistance to this class of antibiotics is one criterion for defining extensively drug resistant tuberculosis. Fluoroquinolones are also used for drug-susceptible tuberculosis in patients unable to tolerate first-line agents. Widespread Fluoroquinolone use in the community has resulted in Fluoroquinolone resistance in Mycobacterium tuberculosis. Despite this, Fluoroquinolone drug susceptibility testing is rarely performed in non- multidrug-resistant TB. The existing global evidence suggests that FQ resistance remains largely confined to MDR-TB strains. Currently there are many phenotypic and Genotpypic methods available for determining resistance to Fluoroquinolone. However, some of these techniques involve expensive equipment and infrastructure. Hence, in the current study, different methods were explored and the most reliable and feasible techniques in Mycobacterial laboratories have been discussed.