Bhaskara Rao K-V

Department of Bio-Medical Sciences

School of Bio Sciences and Technology

Vellore Campus

Hemath naveen k s

Gaurav kumar karthik l

Fulltext

Vellore Institute of Technology (VIT) is a private university located in&nbsp;Tamil Nadu, India. Founded in 1984, as Vellore Engineering College, the institution offers 20 undergraduate, 34 postgraduate, four integrated and four research programs. It has campuses in Vellore, Amravati, Bhopal and Chennai.

VIT is one of the top ranked private universities in India according to NIRF, THE and QS Rankings.&nbsp;Govt. of India has recognized&nbsp;VIT, Vellore as an&nbsp;Institution of Eminence. This has allowed VIT to take independent quality initiatives and move up in world ranking.

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VIT University

Scientia Iranica

Green Synthesis, Characterization and Applications of Nanoparticles

Broad spectrum antibacterial silver nanoparticle green synthesis: Characterization, and mechanism of action

Objectives: The aim of the present study was to evaluate the anticandidal activity of biologically synthesized silver nanoparticles using the culture filtrate of Streptomyces sp.VITPK1. Materials and methods: Silver nanoparticles were synthesized using the culture filtrate of Streptomyces species isolated from brine spring located at Thoubal District, Manipur, India. The isolate was identified by molecular taxonomic characterization and designated as Streptomyces sp.VITPK1. The synthesized silver nanoparticles (AgNPs) were characterized by UV-visible spectra, X-ray diffraction (XRD) patterns, Energy Dispersive Analysis of X-rays (EDAX) and Fourier Transform Infrared (FTIR) analysis. The antifungal activity of the synthesized silver nanoparticles was evaluated against selected Candida species. Results: The synthesized AgNPs showed a surface plasmon resonance peak at 425. nm. XRD patterns showed the crystalline peaks at 38.15° (111), 44.35° (200), 64.52° (220) and 77.49° (311) matching with the diffraction facets of silver. The size of the AgNPs was in the range of 20-45. nm. The EDAX analysis revealed the presence of silver as the major metal in the sample. The synthesized AgNPs showed anticandidal activity against Candida albicans, Candida tropicalis and Candida krusei with a maximum zone of inhibition of 20. mm against C.albicans. Conclusions: The results of this study suggest that the green synthesis of silver nanoparticles using Streptomyces sp.VITPK1 have the ability to act against pathogenic Candida strains. © 2014 Elsevier Masson SAS.

Journal de Mycologie Médicale

Anticandidal activity of silver nanoparticles synthesized using Streptomyces sp.VITPK1

Emergence of antibiotic resistance by bacteria has become a serious threat for public health worldwide. In this study, Streptomyces isolated from fertile soil sample was tested for biosynthesis of silver nanoparticles (AgNps) using cell-free supernatant and synthesized AgNps were screened for anti-ESBL (extended spectrum b-lactamase) activity against multi-drug resistant (MDR) ESBL-producing strain Klebsiella pneumoniae (ATCC 700603) and other medically important pathogens. Synthesis of AgNps was confirmed by change in pale yellow color to dark brown color and characteristic absorption spectra at 420 nm. The XRD spectrum displayed typical peaks of crystalline silver and EDAX analysis showed a major signal for silver. FTIR spectra revealed prominent peaks at 3,294 cm-1 (NH stretching due to amide group), 2,952 cm-1 (aldehydic C-H stretching) 1,658 cm-1 indicating the presence of carbonyl group. AgNps were spherical in shape with size ranging from 20 to 70 nm. The synthesized AgNps showed significant antimicrobial activity against standard ESBL pathogen K. pneumoniae (22 mm), 21 mm against clinical ESBL isolate E. coli and 16 mm against clinical ESBL isolates K. pneumoniae and Citrobacter species, respectively. The results of this study suggest that AgNps synthesized by Streptomyces sp. VITSJK10 can be used as a potential alternative to control MDR ESBL pathogens. The present study aimed for green synthesis of AgNps using Streptomyces species and to explore its anti-ESBL activity. © Springer-Verlag 2013.

Bioprocess and Biosystems Engineering

Anti-ESBL activity of silver nanoparticles biosynthesized using soil Streptomyces species

Silver nanoparticles (AgNPs) have application potential in diverse areas ranging from wound healing to catalysis and sensing. The possibility for optimizing the physical, chemical and optical properties for an application by tailoring the shape and size of silver nanoparticles<?index value="Silver nanoparticles: has motived much research on methods for synthesis of size- and shape-controlled AgNPs. The shape and size of AgNPs are reported to vary depending on choice of the Ag precursor salt, reducing agent, stabilizing agent and on the synthesis technique used. This chapter provides a detailed review on various synthesis approaches that may be used for synthesis of AgNPs of desired size and shape. Silver nanoparticles may be synthesized using diverse routes, including, physical, chemical, photochemical, biological and microwave -based techniques. Synthesis of AgNPs of diverse shapes, such as, nanospheres, nanorods, nanobars, nanoprisms, decahedral nanoparticles and triangular bipyramids is also discussed for chemical-, photochemical- and microwave-based synthesis routes. The choice of chemicals used for reduction and stabilization of nanoparticles is found to influence their shape and size significantly. A discussion on the mechanism of synthesis of AgNPs through nucleation and growth processes is discussed for AgNPs of varying shape and sizes so as to provide an insight on the various synthesis routes. Techniques, such as, electron microscopy, spectroscopy, and crystallography that can be used for characterizing the AgNPs formed in terms of their shape, sizes, crystal structure and chemical composition are also discussed in this chapter. Graphical Abstract: © 2019 2019 Walter de Gruyter GmbH, Berlin/Boston.

Physical Sciences Reviews

Synthesis and characterization of size- And shape-controlled silver nanoparticles

Journal of Human, Environment, and Health Promotion

Keratin Production by Decomposing Feather Waste Using Some Local Bacillus spp. Isolated from Poultry Soil

Iranian Journal of Virology

Rotavirus NSP4 protein as a viral biotoxin selectively promotes cytotoxicity

Process Biochemistry

Extracellular synthesis of antibacterial silver nanoparticles using psychrophilic bacteria

ACS Nano

Directed Self-Assembly of Nanoparticles

Journal of Experimental Nanoscience

Self-assembly of silver nanoparticles into dendritic flowers from aqueous solution

Extracellular biosynthesis of silver nanoparticles using cell filtrate of Streptomyces sp. ERI-3

Journal	Data powered by TypesetScientia Iranica
Publisher	Data powered by TypesetElsevier BV
ISSN	1026-3098
Open Access	0