The objective of this article is to deal with the application of nanoparticles synthesized using Ag 2 O and Cu 2 O were doped with TiO 2 . The nanoparticles were synthesized using Avocado fruit extract and their applications were evaluated for antimicrobial with cytotoxic properties. The TiO 2 doped with Ag 2 O and Cu 2 O nanoparticles were characterized using UV-Vis spectrum, Transmission Electron Microscopy (TEM), Fourier Transform Infrared Spectroscopy (FTIR), Elemental X-ray Analysis (EDAX) and X-ray Diffractometer (XRD) patterns respectively. The both Ag 2 O and Cu 2 O were doped with TiO 2 nanoparticles were in spherical having size ranging from 45-50 nm, and crystalline in nature. Interestingly, the synthesized material exhibited promising activity against the bacterial pathogens such as Escherichia coli, Pseudomonas aeroginosa, Staphylococcus aureus and Salmonella typhi respectively. In addition, the LC50 value (3.929 mg/ml; with 3.102-5.789 as upper and lower limits respectively) against the cell lines were its advantages. Over all, it is concluded that the synthesized material can be used for better biological applications. © 2018 American Scientific Publishers All rights reserved.