Glucose biosensors are used to detect the glucose concentration in a sample. In the first generation amperometric biosensor, an enzyme glucose oxidase is used which is reduced on reacting with glucose yielding Hydrogen Peroxide as a product which then breaks down to Water and Oxygen. The latter reaction is studied by electrolysis. Two electrons are released in the process which produces current. The amount of current produced by the system and due to the release of the electrons is detected by an ammeter. This is directly proportional to the concentration of glucose present. Glucose measurements are commonly based on interactions with one of three enzymes: hexokinase, glucose oxidase (GOx) or glucose-1-dehydrogenase (GDH). A linear graph plots the current and voltage. All the values were plotted in the form of a tabular column and these values were obtained by using Faraday’s law of electrolysis and efficiency equations. The efficiency was shown in the tabular column with the first trial (V=1) showing maximum efficiency. © 2016, Global Research Online. All rights reserved.