Objective: To analyze the yellow aromatic oil obtained from the seeds of Lawsonia inermis by using GC/MS. Methods: GC/MS analysis of henna seed oil was performed using JEOL GCMS-Mate-II model gas chromatograph-mass spectrometer. Data handling was made through JEOL software and matched with NIST library. Results: The GC/MS of the oil sample showed five major peaks corresponding to five compounds, prominent of which were 8-Octadecenoic acid, methyl ester (45.6%) and Pentadecanoic acid, 6-oxo, methyl ester (28.6%). Conclusion: GC/MS of the seed oil resulted in the identification of five compounds which were not reported in literature previously.