Genetic divergence is a measure of selection of parents to produce potential hybrids and for isolation of transgressive segregants from hybrids in further filial generations. The objective of this study was to discriminate between 33 eggplant genotypes using multivariate analysis. The coefficient of variation was high for total phenols content and moderate in fruit yield per plant. Principal component analysis indicated that the first 6 components with an eigen value > 2 contributed about 80.61% of total variability. Magnitudes of the total variance attributable to the first 6 principal components were 32.74, 13.87, 10.68, 8.51, 5.52 and 4.79%, respectively. Flowering time, fruit circumference, fruit width, total phenols content, internodal length, leaf area index, fruit yield per plant and average fruit weight were traits contributing the most to the total variability. Fruit yield per plant, the most important economic trait, exhibited positive, significant, association with fruit circumference (0.557), fruit width, fruit borer infestation, total phenols content and average fruit weight. The distribution of genotypes from different geographical locations into 10 clusters was random, indicating geographical separation may not be the only factor causing genetic diversity. Cluster I contained the most genotypes (15) followed by cluster IX (5) and the minimum number of genotypes were in clusters II, V, VII and X. Intermating between genotypes of clusters I and IX would produce more desirable transgressive segregants for breeding. © Society for the Advancement of Breeding Research in Asia and Oceania (SABRAO) 2016.