Silver nanoparticles (AgNPs) are extensively used in most of the consumer products. To study the genotoxic effects of AgNP to human peripheral blood lymphocytes at lower concentrations is the objecqtive of the present study. We studied the genotoxic effect by lymphocyte proliferation assay and hemolysis assay. Metal ion analysis was also done to check the uptake of nanoparticles in the cell pellet of human peripheral blood cells to ensure the generation of ROS, a possible mechanism by which the DNA damage could happen. The results indicate that the aberrant cell damage for the exposure period of 24hrs was found to be 0, 8 and 24% for 5, 15 and 25μg/mL of AgNPs respectively. Interestingly, cytotoxicity studies on erythrocytes also reveal the toxic effect of silver nanoparticles with membrane damage of the lipo-polysaccharide layer through haemolysis assay. Here we report that AgNPs exhibit geno-toxicity in human peripheral blood lymphocytes through the fragmentation of DNA and other chromosomal aberrations. Ag+ ion and AgNP concentration were analysed at 15μg/mL of AgNP by Atomic absorption spectroscopy (AAS) and was estimated to be 0.725±0.02 μg/mL and 1.859±0.03 μg/mL in culture medium and cell pellet respectively. Taken together, silver nanoparticles are found to be cytotoxic and genotoxic even at a lower concentration such as 1.8μg/mL.