The recent advancements in the field of nanotechnology leads to the productivity of wide range of nano scale products in many fields. Hemidesmus indicus is commonly known as Indian Sarsaparilla which was well explored as an antidiabetic, antitumor, antimicrobial, antioxidant and so on. The present research work focuses on the synthesis of copper (Cu) nanoparticles using the medicinal plant Hemidesmus indicus which was characterized by various preceding analytical tools and its antibacterial properties were investigated against bacterial pathogens. The green route of copper (Cu) nanoparticles synthesis from the root extracts were done by using standard protocols with the time duration of 9-12 hrs. Characterization studies were done by using UV-VIS spectroscopy, Fourier Transform Infra-Red (FTIR) Spectrum, X-Ray Diffractometer (XRD), Scanning electron microscope (SEM), Energy Dispersive X-Ray Spectroscopy Analysis (EDAX) and Dynamic Light Scattering Analysis (DLS). Antibacterial activity against bacterial pathogens was done by agar well diffusion method. The synthesized Cu nanoparticles have a spherical shaped structure figured out from the XRD studies. The Structure morphology was elucidated with the SEM analysis. The FTIR results shows the functional groups present in it and DLS gives the stability of the Cu nanoparticles. The EDAX studies showed the potential compounds capped on the surface of the Cu nanoparticles and it has exhibited a potential antibacterial agent against all the tested strains. The synthesized Cu nanoparticles can be used for its biomedical applications and in the field of nanomedicine. © 2018 E-flow PDF World Research Association. All rights reserved.