We report on silver nanoparticles synthesize with the Corallocarpus epigaeus rhizome extract and their characteristics. The synthesized phytochemical compound acts as both a stabilizing and a reducing agent. We systematically characterize the synthesized silver nanoparticles. UV-Vis spectra confirmed the formation of green synthesized silver nanoparticles from the Corallocarpus epigaeus rhizome. Scanning electron microscopy and high-resolution transmission electron microscopy images exhibited indicated spherical and randomly distributed silver nanoparticles. The synthesized nanoparticles of the Corallocarpus epigaeus rhizome aqueous extract showed bacteriostatic activity against Pseudomonas aeuginosa, Escherichia coli, Proteus mirabilis, Bacillus cereus, and Staphylococcus aureus. The synthesized silver nanoparticles from Corallocarpus epigaeus rhizome aqueous extract displayed fungicidal activity of Mucor species, Aspergillus niger, Aspergillus flavus, and Rhizopus species. Furthermore, the silver nanoparticles exhibited a cytotoxic activity of inhibiting HepG2 cell proliferation in the MTT cytotoxicity test. © 2021, Korean Vacuum Society. All rights reserved.