The residual TC in the aquatic system is a major threat to human and animal lives. In this study, green synthesized bimetallic Fe/Pd and in-situ clay supported Fe/Pd was used for the efficient removal of tetracycline antibiotics from the aqueous system. Green synthesized bimetallic Fe/Pd nanocomposite and in-situ clay supported Fe/Pd (B-Fe/Pd) were characterized by SEM-EDX, FTIR, and XRD to confirm their size and surface functional groups. The batch removal processes were performed to examine the effect of operational parameters such as dopant Pd concentration (5-20mM), sorbents: Fe/Pd (100-1000mg/L) and B-Fe/Pd (100-300mg/L), pollutant TC (20-60mg/L) and pH (3-9) on the TC removal. At the optimized conditions, Fe/Pd (sorbent dosage 1000mg/L, pH 7, TC conc. 20mg/L), and B-Fe/Pd (sorbent dosage 300mg/L, pH 7, TC conc. 20mg/L) nanocomposite can remove 81 % and 97 % of TC from aqueous sample. Higher surface area and prolonged shelf-life of B-Fe/Pd with highest removal percentage make it more effective and efficient material for TC removal; further, the statistical optimization of TC removal by B-Fe/Pd was carried out by response surface methodology, and 95 % of TC could be eliminated from the aqueous solution. Reusability studies of B-Fe/Pd showed that the TC removal efficiency up to 3rd cycle with above 60 % TC removal and the validation on real water sample confirmed with above 75 % TC removal. Residual toxicity of B-Fe/Pd interacted TC solution on freshwater chlorella sp. showed less toxicity, which indicates the environmentally friendly and hazardous free nature of B-Fe/Pd. © 2020 Elsevier Ltd.