Luminescence is the property of light emission. If this light emission is emitted by living organism then this phenomenon is known as the Bioluminescence “the living light”. We may feel wonderful that most of the deep sea (ocean) living organisms are having their own physiological process for light production. The present experiment aims to study the method of staining and biochemical analysis of the bioluminescent bacteria that are present in shiny scales of fish (selected species Indian mackerel, Rohu, Black-spot Snapper, Fin bream & Indian Goat Fish from Vellore Fish market From the staining and other analytical techniques the different ranges of light intensities are noted so the biochemical, molecular characterization is done. Thus they are identified if present in scales. From the various Staining methods the obtained results are encrypted. © RJPT All right reserved.