The current work on Yellow Ornamental Lantana flowers focuses on investigating its possible antimicrobial properties, presence of active alkenes and long chain fatty acid molecules. It was observed to have effective antimicrobial activity against S. aureus, P. mirabilis, P. aeruginosa, B. cereus and E. coli. The presence of Erucamide and Cis-5, 8,11,14,17-eicosapentaenoic acid, tertbutyldimethylsilyl ester (a derivative of omega-3- fatty acid, eicosapentaenoic acid) was reported by the GC-MS analysis of the methanolic extract of the flowers.