Modified Gabor-Wavelet Transform (MGWT) has been previously used for identification of protein coding regions. This method does not significantly suppress the non-coding regions which may result incorrect identification. To enhance the discrimination between the coding and non-coding regions of the DNA sequence, Signal Boosting Technique (SBT) is applied on the projection coefficients which are obtained by applying MGWT. The performance of the proposed Modified Gabor-Wavelet Transform with Signal Boosting Technique (MGWT-SBT) is compared with other existing methods such as MGWT, Short-Time Discrete Fourier Transform (ST-DFT), anti-notch filter and multi-stage filter. Results show that the proposed method gives better identification accuracy as compared to other methods. Copyright © 2010 Inderscience Enterprises Ltd.